| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.255454 |
| Chromosome: | chromosome 9 |
| Location: | 5121937 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g398808 | (1 of 2) PF01465 - GRIP domain (GRIP) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAGCAGCGCCAGCCCACGACGCACGGAACA |
| Internal bar code: | GTACCGTCGCCGGACGTTACGGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 59 |
| LEAP-Seq percent confirming: | 96.9925 |
| LEAP-Seq n confirming: | 129 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCCATCTTCTCATTCGCTTC |
| Suggested primer 2: | GGAGAAGTGGAGGCAGTCAG |