| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.255461 |
| Chromosome: | chromosome 6 |
| Location: | 7378654 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g299300 | TUG,TUG1 | Gamma tubulin; (1 of 1) K10389 - tubulin gamma (TUBG) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCCAGGGGCAAAATTCCTGGCGACATCACA |
| Internal bar code: | TATCGAGCTGTGTGGATCACTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 285 |
| LEAP-Seq percent confirming: | 98.8227 |
| LEAP-Seq n confirming: | 6883 |
| LEAP-Seq n nonconfirming: | 82 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGTATGGAAAGCCTGACCAA |
| Suggested primer 2: | GTATACGGTCCCGCGTCTTA |