Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | + |
Strain: | LMJ.RY0402.255506 |
Chromosome: | chromosome 3 |
Location: | 6300018 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre03.g193250 | (1 of 1) 2.7.1.160 - 2'-phosphotransferase / Yeast 2'-phosphotransferase | intron |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTGCGGTGTCAGTGGGCATGCAACCATGC |
Internal bar code: | GTTAGCTACGGTGCACTTGGAC |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 301 |
LEAP-Seq percent confirming: | 99.0586 |
LEAP-Seq n confirming: | 947 |
LEAP-Seq n nonconfirming: | 9 |
LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | TCAGCTTCGAACCTTGACCT |
Suggested primer 2: | GCACCTTCTCAGTACTCGCC |