Insertion junction: LMJ.RY0402.255506_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.255506
Chromosome:chromosome 3
Location:6300018
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre03.g193250 (1 of 1) 2.7.1.160 - 2'-phosphotransferase / Yeast 2'-phosphotransferase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTTGCGGTGTCAGTGGGCATGCAACCATGC
Internal bar code:GTTAGCTACGGTGCACTTGGAC

Confirmation - LEAP-Seq

LEAP-Seq distance:301
LEAP-Seq percent confirming:99.0586
LEAP-Seq n confirming:947
LEAP-Seq n nonconfirming:9
LEAP-Seq n unique pos:8

Suggested primers for confirmation by PCR

Suggested primer 1:TCAGCTTCGAACCTTGACCT
Suggested primer 2:GCACCTTCTCAGTACTCGCC