| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.255506 |
| Chromosome: | chromosome 3 |
| Location: | 6300024 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g193250 | (1 of 1) 2.7.1.160 - 2'-phosphotransferase / Yeast 2'-phosphotransferase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGCAACCCCGTACTGCTGCCCCATCCACGA |
| Internal bar code: | CCAAACTCTATCCTACTTCAAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 391 |
| LEAP-Seq percent confirming: | 99.7344 |
| LEAP-Seq n confirming: | 3379 |
| LEAP-Seq n nonconfirming: | 9 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCAGCTTCGAACCTTGACCT |
| Suggested primer 2: | GCACCTTCTCAGTACTCGCC |