| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.255508 |
| Chromosome: | chromosome 17 |
| Location: | 1695459 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g708550 | HTA20,HTA27 | Histone H2A; (1 of 30) K11251 - histone H2A (H2A) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGAGAGTAGCGAGGTCAAATTGTGCAAGT |
| Internal bar code: | GTGACAGCGATCACAGGGCGAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 618 |
| LEAP-Seq percent confirming: | 98.8406 |
| LEAP-Seq n confirming: | 682 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 16 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACTCGGTACAATGGCTGGTC |
| Suggested primer 2: | GTGACATAGGCTGACGGGTT |