| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.255538 |
| Chromosome: | chromosome 11 |
| Location: | 3681269 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g482600 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGTGATGGCGGTGTGGATCCGGGATCCCCA |
| Internal bar code: | GTATTAGCTGGCTCATACACGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 533 |
| LEAP-Seq percent confirming: | 85.1462 |
| LEAP-Seq n confirming: | 728 |
| LEAP-Seq n nonconfirming: | 127 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTCAAGCCCTCTGTCCTACG |
| Suggested primer 2: | AGGAGGAGTCGGAGAAGGAG |