| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.255552 |
| Chromosome: | chromosome 3 |
| Location: | 3612180 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g168550 | AOT3 | Amino acid transporter; (1 of 1) K15015 - solute carrier family 32 (vesicular inhibitory amino acid transporter) (SLC32A, VGAT) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGAGATGTGCCGGTAATGGTTTTGTAGCTG |
| Internal bar code: | GCGGATCTCGCTCGTCCCCGCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 337 |
| LEAP-Seq percent confirming: | 98.3828 |
| LEAP-Seq n confirming: | 365 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTCTTGACAGGTGCGTCTGA |
| Suggested primer 2: | GTGGAAGTGGAGATGGAGGA |