| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.255559 |
| Chromosome: | chromosome 17 |
| Location: | 3295230 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g723150 | ERO1 | (1 of 1) K10950 - ERO1-like protein alpha (ERO1L); Endoplasmic reticulum oxidoreductin | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCAGGTGAGGCCGGGGGCCGGCGGGGCCG |
| Internal bar code: | ACGCGTAGCGGCCCTCTCCGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 143 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 8 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGGTGTGTGTGTGTGTGTGT |
| Suggested primer 2: | TGGTCGAAGCATCGTTGATA |