| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.255572 |
| Chromosome: | chromosome 4 |
| Location: | 1801666 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g211900 | ELG35,ELG37 | Exostosin-like glycosyltransferase 37; (1 of 34) 2.4.2.41 - Xylogalacturonan beta-1,3-xylosyltransferase / Xylogalacturonan xylosyltransferase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTCCATCATGCCGCGCCCACACAGCTCCAA |
| Internal bar code: | ATTCTCATGGGAGCCGAAGCGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 416 |
| LEAP-Seq percent confirming: | 93.9297 |
| LEAP-Seq n confirming: | 294 |
| LEAP-Seq n nonconfirming: | 19 |
| LEAP-Seq n unique pos: | 14 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATGTTGGGACAAGCTTCCAC |
| Suggested primer 2: | CAGGGACTAGTATCGCGAGC |