Insertion junction: LMJ.RY0402.255581_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.255581
Chromosome:chromosome 5
Location:3347290
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre05.g240700 FAP378 Flagellar Associated Protein 378; (1 of 1) PF00530//PF05572 - Scavenger receptor cysteine-rich domain (SRCR) // Pregnancy-associated plasma protein-A (Peptidase_M43) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTGGTGGACTGGCCGCGTTCCATCAACGTG
Internal bar code:GAACAGCTATAAGCGCGAGGCC

Confirmation - LEAP-Seq

LEAP-Seq distance:228
LEAP-Seq percent confirming:99.5074
LEAP-Seq n confirming:202
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:8

Suggested primers for confirmation by PCR

Suggested primer 1:TATGCTGTACTGGGGAAGCC
Suggested primer 2:TCATTCTTAAGCCCGGATTG