| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.255638 |
| Chromosome: | chromosome 6 |
| Location: | 8653332 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g309450 | CPZ | (1 of 1) 3.4.17.12 - Carboxypeptidase M | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CATGAACCACCCCAGCATGCGCTTCAGCTG |
| Internal bar code: | GAGTCTTCTTCAATGCTGTTTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 279 |
| LEAP-Seq percent confirming: | 92.6966 |
| LEAP-Seq n confirming: | 165 |
| LEAP-Seq n nonconfirming: | 13 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTTGACGGTTAGGCTCTGCT |
| Suggested primer 2: | TACCGCTTTTCCTCACATCC |