| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.255639 |
| Chromosome: | chromosome 7 |
| Location: | 3774854 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g338250 | (1 of 4) PF04073 - Aminoacyl-tRNA editing domain (tRNA_edit) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCTTTCCTGCGGCCCCCAGTCCCTGCGCT |
| Internal bar code: | CAACGGTGTCGTCAAGTGGGGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 482 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 203 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CATGAGCGTCAAGGAGTTCA |
| Suggested primer 2: | ACGGTAGGTGCCCTAGTGTG |