| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.255682 |
| Chromosome: | chromosome 3 |
| Location: | 6672698 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g197350 | CDC5 | (1 of 1) K12860 - pre-mRNA-splicing factor CDC5/CEF1 (CDC5L, CDC5, CEF1); Cell Division Cycle 5 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGAGCGCTCCTGCAGGTCCACCTCGCGCC |
| Internal bar code: | GGTTCTATCGAATCTTATGCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 164 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 79 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTCGGTTGGAAGCGTTATGT |
| Suggested primer 2: | ATTGGAATGAGACCACTCCG |