| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.255699 |
| Chromosome: | chromosome 12 |
| Location: | 5711783 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g532700 | (1 of 1) IPR000104//IPR007308 - Antifreeze protein, type I // Protein of unknown function DUF408 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TACTCCGGTTGCGTACTAGGATGCGCCAAC |
| Internal bar code: | GTCTGCCCGAGGGGCCCCGACA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 546 |
| LEAP-Seq percent confirming: | 99.5162 |
| LEAP-Seq n confirming: | 4731 |
| LEAP-Seq n nonconfirming: | 23 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGGACTTCTAGACAGCAGGG |
| Suggested primer 2: | CATGGTGGACTGAACGTCAC |