| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.255702 |
| Chromosome: | chromosome 2 |
| Location: | 5373568 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g107213 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCATTACGGATTGGTTTGTTGGCTGCTGTG |
| Internal bar code: | GGCAAGGCTTGGGGGCGCGGAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 492 |
| LEAP-Seq percent confirming: | 98.1366 |
| LEAP-Seq n confirming: | 316 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AATCCTTTGATATCCGCACG |
| Suggested primer 2: | TACAGCACCCGACCTAAACC |