Insertion junction: LMJ.RY0402.255725_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.255725
Chromosome:chromosome 17
Location:6240188
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre17.g742250 NCG9,CDKG2 Cyclin dependent kinase 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTATAACGAGTGATGCTTGCGCTGCTGTCT
Internal bar code:TCAAACGTCAGTTTCATGACCC

Confirmation - LEAP-Seq

LEAP-Seq distance:1044
LEAP-Seq percent confirming:98.2955
LEAP-Seq n confirming:173
LEAP-Seq n nonconfirming:3
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:TCTCTGTATCCCAACCCCTG
Suggested primer 2:ACAGGTGGTGACGCTCTTCT