| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.255726 |
| Chromosome: | chromosome 10 |
| Location: | 4917364 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g454850 | (1 of 6) PF07716 - Basic region leucine zipper (bZIP_2) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCACACGAATGGCGATCGCATCTAAGCGCC |
| Internal bar code: | ACCGTCTAGATCGCCAATTGAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 202 |
| LEAP-Seq percent confirming: | 92.4528 |
| LEAP-Seq n confirming: | 49 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGCTACAGCCTCCACTTCC |
| Suggested primer 2: | TTTGGTTTAGTTTGGGCTGG |