| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.255770 |
| Chromosome: | chromosome 6 |
| Location: | 3856548 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g278175 | (1 of 1) K14793 - ribosomal RNA-processing protein 9 (RRP9) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCCTGCAAACAGCAGGAGAAATGCCCATGT |
| Internal bar code: | CGCGATGTAACTGGAAGTTGTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 817 |
| LEAP-Seq percent confirming: | 99.5696 |
| LEAP-Seq n confirming: | 694 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCAACATATGGGTGTGGGTG |
| Suggested primer 2: | AGTCGAGTTTGCCATTTGCT |