| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.255792 |
| Chromosome: | chromosome 3 |
| Location: | 7299632 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g206950 | (1 of 1) PF05183 - RNA dependent RNA polymerase (RdRP) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGACACTCATTCATCCTCCCATTGCCTAC |
| Internal bar code: | TTGCACTTCCGGCGCACATATG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 626 |
| LEAP-Seq percent confirming: | 99.5305 |
| LEAP-Seq n confirming: | 212 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATGTGCCTCGGTGTGTGTTA |
| Suggested primer 2: | GCTACATGAGGGCTTTCGAG |