| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.255793 |
| Chromosome: | chromosome 13 |
| Location: | 3094234 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g584750 | NDUFAF5,SMM46 | (1 of 1) K18162 - NADH dehydrogenase [ubiquinone] 1 alpha subcomplex assembly factor 5 [EC:2.1.1.-] (NDUFAF5); NADH:ubiquinone oxidoreductase 1 alpha subcomplex assembly factor 5 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGGAAGGTATCCTTGCCAGCCGGGTGCAC |
| Internal bar code: | TCACGTCCGCGGAAAGGCAGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 343 |
| LEAP-Seq percent confirming: | 99.5945 |
| LEAP-Seq n confirming: | 5403 |
| LEAP-Seq n nonconfirming: | 22 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TAGCGCGCACATTTTGTAAG |
| Suggested primer 2: | AGTACATAACCCTGCGGACG |