Insertion junction: LMJ.RY0402.255825_3

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.255825
Chromosome:chromosome 17
Location:2809753
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre17.g718900 FAP88 Flagellar Associated Protein 88; (1 of 1) PTHR23237//PTHR23237:SF6 - NUCLEOLAR PROTEIN FAMILY A MEMBER 1 SNORNP PROTEIN GAR1 // H/ACA RIBONUCLEOPROTEIN COMPLEX SUBUNIT 1 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AGCCTCGACCCGACGTCCACACGTCCCCAC
Internal bar code:ATATCGGTCGATGGGCTCATGC

Confirmation - LEAP-Seq

LEAP-Seq distance:320
LEAP-Seq percent confirming:92.053
LEAP-Seq n confirming:139
LEAP-Seq n nonconfirming:12
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:GGTGTGTGTCAAACCGTCAG
Suggested primer 2:ACAGCTACACCCATTTTCGG