| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.255825 |
| Chromosome: | chromosome_17 |
| Location: | 2809753 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre17.g718900 | FAP88 | Flagellar Associated Protein | antisense | intron |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | AGCCTCGACCCGACGTCCACACGTCCCCAC |
| Internal bar code: | ATATCGGTCGATGGGCTCATGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 320 |
| LEAP-Seq percent confirming: | 92.053 |
| LEAP-Seq n confirming: | 139 |
| LEAP-Seq n nonconfirming: | 12 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGTGTGTGTCAAACCGTCAG |
| Suggested primer 2: | ACAGCTACACCCATTTTCGG |