| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.255836 |
| Chromosome: | chromosome 14 |
| Location: | 831535 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g613200 | Putative metallochaperone; (1 of 5) PTHR13748//PTHR13748:SF4 - COBW-RELATED // COBW DOMAIN-CONTAINING PROTEIN DDB_G0274527 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGGATCGGTGTGGATCCAATGGACGAGAC |
| Internal bar code: | GCCTTATTTCGTGGAAACTTTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 391 |
| LEAP-Seq percent confirming: | 99.7702 |
| LEAP-Seq n confirming: | 4776 |
| LEAP-Seq n nonconfirming: | 11 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGTTCAGCTCTACGCCATTG |
| Suggested primer 2: | ATGTGTGTGTTGTGTGCGTG |