| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.255889 |
| Chromosome: | chromosome_2 |
| Location: | 5786786 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre02.g110750 | LHCB7 | Chlorophyll a/b binding protein | sense | CDS |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | CGGCGCATCGGCCGGGGCTGGCTGCTCAGA |
| Internal bar code: | TTCCCGCCGGGATCCGCCTCAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 268 |
| LEAP-Seq percent confirming: | 99.5575 |
| LEAP-Seq n confirming: | 225 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTTTCCCAAGCTGTCCATGT |
| Suggested primer 2: | GGACTTGCAGAAACTGCTCC |