Insertion junction: LMJ.RY0402.255891_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.255891
Chromosome:chromosome 17
Location:5946401
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre17.g740261 FAP365 (1 of 96) IPR019734 - Tetratricopeptide repeat; Flagellar Associated Protein 365 5'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTCTCTTGGCCTGTATCAATGTGCTATTCG
Internal bar code:GCCAGCGCTGGCGTACCGACGC

Confirmation - LEAP-Seq

LEAP-Seq distance:323
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:259
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:5

Suggested primers for confirmation by PCR

Suggested primer 1:ATCCGACACCACACTGAACA
Suggested primer 2:CGCGTGCACACTTCATAGTT