| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.255907 |
| Chromosome: | chromosome 1 |
| Location: | 5322844 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g037300 | (1 of 140) IPR002048 - EF-hand domain | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACTAGCTGTGGCAAGGCCGCTGGCCCGTAC |
| Internal bar code: | GTTGGGGGAGTCAATCTTCTGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 330 |
| LEAP-Seq percent confirming: | 92.9078 |
| LEAP-Seq n confirming: | 262 |
| LEAP-Seq n nonconfirming: | 20 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTCTCCTTCTACCGCTCCCT |
| Suggested primer 2: | AACAAAAATGAGCTCCACCG |