| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.255942 |
| Chromosome: | chromosome 10 |
| Location: | 3918605 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g448150 | (1 of 6) PF01847 - von Hippel-Lindau disease tumour suppressor protein (VHL) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGTTTTTGTGGAATGGGGTCAAGAAAGCTT |
| Internal bar code: | ACGGCGGACTCTAGCAATGCCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 333 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 23 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTTCGGGCGTCATTGTAACT |
| Suggested primer 2: | TTCGCGTGAATCTTGTCTTG |