Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | + |
Strain: | LMJ.RY0402.255956 |
Chromosome: | chromosome_8 |
Location: | 4886793 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre08.g384900 | sense | intron |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | GTGTGGCAAAGCGTGGGCGCGGGCTGCAGG |
Internal bar code: | GGCCGTGTTCCGTGAGTGCTGA |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 368 |
LEAP-Seq percent confirming: | 96.3576 |
LEAP-Seq n confirming: | 291 |
LEAP-Seq n nonconfirming: | 11 |
LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GAGCTGAAGAGGCGAGCTTA |
Suggested primer 2: | CACATGTCGTTGGTACCCTG |