| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.255960 |
| Chromosome: | chromosome 13 |
| Location: | 2880540 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g583450 | EZY17 | Early zygote expressed protein; (1 of 6) PTHR15907:SF21 - DUF614 FAMILY PROTEIN | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGGATTGTAGTGCGGTGCTGTGGAAACGA |
| Internal bar code: | GCTTCGACCCAACGTTATGGCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 29 |
| LEAP-Seq percent confirming: | 43.75 |
| LEAP-Seq n confirming: | 7 |
| LEAP-Seq n nonconfirming: | 9 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGTACTCAGGTCGATGGGGT |
| Suggested primer 2: | TCATGCGCTGTTCTTCAAAC |