| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.255965 |
| Chromosome: | chromosome 1 |
| Location: | 2669350 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g015600 | (1 of 1) PTHR23346//PTHR23346:SF7 - TRANSLATIONAL ACTIVATOR GCN1-RELATED // TRANSLATIONAL ACTIVATOR GCN1 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAGGGGCACATGCGTTTGCGTGCATCCATC |
| Internal bar code: | AGCGGCCGACCGCGTCACCGCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 175 |
| LEAP-Seq percent confirming: | 98.5423 |
| LEAP-Seq n confirming: | 338 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGTCCTCGTCTGTGAGCTTC |
| Suggested primer 2: | CCTTCCTCAAGTGCCTCAAC |