| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.255998 |
| Chromosome: | chromosome_12 |
| Location: | 5955353 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre12.g534550 | PHR4 | antisense | 3'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | TATATGGTACAATGCAGTCAAAGCTGCTAC |
| Internal bar code: | CCATATTGGCGCGAGTCGAGTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 382 |
| LEAP-Seq percent confirming: | 25.2969 |
| LEAP-Seq n confirming: | 213 |
| LEAP-Seq n nonconfirming: | 629 |
| LEAP-Seq n unique pos: | 23 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CATATAGGTGCTGATGGGGG |
| Suggested primer 2: | TTGTGTTCGAGCTGCTATGG |