Insertion junction: LMJ.RY0402.256018_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.256018
Chromosome:chromosome 6
Location:3335879
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre06.g277450 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CACCCAGCAGTGCCAACCCCTACCCATCCA
Internal bar code:GGAAAACCTCTCTGCCACAGCC

Confirmation - LEAP-Seq

LEAP-Seq distance:38
LEAP-Seq percent confirming:47.8261
LEAP-Seq n confirming:22
LEAP-Seq n nonconfirming:24
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:TGTTGTGATGACGTGTGTGG
Suggested primer 2:AGCGTGTGGACACTAATCCC