| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.256034 |
| Chromosome: | chromosome 10 |
| Location: | 4560530 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g452450 | TIC110 | Chloroplast envelope protein; (1 of 1) PF16940 - Chloroplast envelope transporter (Tic110) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCTCCCGAATGCACTGATGCACCTAGCAAG |
| Internal bar code: | TTTTCGTTCCTTCTCGCTCTCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 625 |
| LEAP-Seq percent confirming: | 99.5807 |
| LEAP-Seq n confirming: | 6175 |
| LEAP-Seq n nonconfirming: | 26 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTGGGAGCTTAGTAGGGCT |
| Suggested primer 2: | TCTACGTCGCAGGACATCTG |