| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.256064 |
| Chromosome: | chromosome 11 |
| Location: | 2022551 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g468383 | (1 of 1) PTHR22844//PTHR22844:SF176 - F-BOX AND WD40 DOMAIN PROTEIN // SUBFAMILY NOT NAMED | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTTTTCGTTAGGTTGATTGGAGTTTGGTT |
| Internal bar code: | CCGCCGTGCGATACGACTTAGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 171 |
| LEAP-Seq percent confirming: | 99.8081 |
| LEAP-Seq n confirming: | 520 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGGGTCGGAACTTTGTGTTG |
| Suggested primer 2: | GTTTCAGATGTGCAGGAGCA |