Insertion junction: LMJ.RY0402.256067_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.256067
Chromosome:chromosome 2
Location:2753473
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre02.g093850 (1 of 1) IPR000104//IPR000595//IPR001611//IPR003591//IPR018490 - Antifreeze protein, type I // Cyclic nucleotide-binding domain // Leucine-rich repeat // Leucine-rich repeat, typical subtype // Cyclic nucleotide-binding-like 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TCATGTCTCTATGTCAGCGGACCTACAACA
Internal bar code:TTCGCCTTCCTCGGGGACGCG

Confirmation - LEAP-Seq

LEAP-Seq distance:633
LEAP-Seq percent confirming:95.2029
LEAP-Seq n confirming:516
LEAP-Seq n nonconfirming:26
LEAP-Seq n unique pos:9

Suggested primers for confirmation by PCR

Suggested primer 1:TTTAGCTGCGTGTGAACTGG
Suggested primer 2:CGCGTATCTCAACTCGTGAA