| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.256067 |
| Chromosome: | chromosome 2 |
| Location: | 2753476 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g093850 | (1 of 1) IPR000104//IPR000595//IPR001611//IPR003591//IPR018490 - Antifreeze protein, type I // Cyclic nucleotide-binding domain // Leucine-rich repeat // Leucine-rich repeat, typical subtype // Cyclic nucleotide-binding-like | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGTGTTGAGCACACATCCTGCTTGCTGTT |
| Internal bar code: | ATTTGTACGCAATACTGTGGCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 484 |
| LEAP-Seq percent confirming: | 99.0588 |
| LEAP-Seq n confirming: | 1263 |
| LEAP-Seq n nonconfirming: | 12 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTTAGCTGCGTGTGAACTGG |
| Suggested primer 2: | CGCGTATCTCAACTCGTGAA |