| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.256073 |
| Chromosome: | chromosome 10 |
| Location: | 2847002 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g439700 | CGL28 | conserved expressed putative RNA binding protein; (1 of 89) PF00076 - RNA recognition motif. (a.k.a. RRM, RBD, or RNP domain) (RRM_1) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAGTAGCGCGCCGCTGCAGTTGAGTGCGGC |
| Internal bar code: | CGGACGCTTGTCGATCGGAGCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1102 |
| LEAP-Seq percent confirming: | 99.6426 |
| LEAP-Seq n confirming: | 9757 |
| LEAP-Seq n nonconfirming: | 35 |
| LEAP-Seq n unique pos: | 14 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CATTGAGTTCATGGACGTGG |
| Suggested primer 2: | AACTGCCAACTACATTGCCC |