| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.256085 |
| Chromosome: | chromosome 1 |
| Location: | 4297851 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g028950 | (1 of 7) 2.8.1.1 - Thiosulfate sulfurtransferase / Thiosulfate thiotransferase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACTTGCCGCTCTTCTCGGTGGGGTACTTGG |
| Internal bar code: | TTCGTATCGCTGAGTCAGTAGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 415 |
| LEAP-Seq percent confirming: | 99.7489 |
| LEAP-Seq n confirming: | 2781 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTTTTGGAGAAGTTGCCTCG |
| Suggested primer 2: | CTGAGTGTAAGGCAGCCACA |