| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.256098 |
| Chromosome: | chromosome 9 |
| Location: | 3875282 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g392023 | CSV2 | Chlamydomonas-specific family protein | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTTCGTGATGGTGTGGTTAACAGGTTATGC |
| Internal bar code: | GGCAAAATGCGGAACATGCACG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 493 |
| LEAP-Seq percent confirming: | 99.9171 |
| LEAP-Seq n confirming: | 3614 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGCTGTACAAGTGCTGAGGG |
| Suggested primer 2: | ACACGGACTCTACCACAGGG |