| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.256101 |
| Chromosome: | chromosome 11 |
| Location: | 1588147 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g467760 | CDS|outside_mRNA |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCAAGGTGCGTGCGGTGCGGCTTGGCGTGC |
| Internal bar code: | GGCCCGACAGCCGTCGTAGTCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 378 |
| LEAP-Seq percent confirming: | 98.8417 |
| LEAP-Seq n confirming: | 512 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGCAGGGACATATCAGTGGG |
| Suggested primer 2: | GAGAGAGCTTGACGGGATTG |