| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.256119 |
| Chromosome: | chromosome 1 |
| Location: | 5389633 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g037950 | (1 of 29) PF13637 - Ankyrin repeats (many copies) (Ank_4) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAAAACTTGTCAATTAAATCCCGTGGAAAT |
| Internal bar code: | TTCTGACGCGGCCGGACTTGCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 450 |
| LEAP-Seq percent confirming: | 91.8182 |
| LEAP-Seq n confirming: | 101 |
| LEAP-Seq n nonconfirming: | 9 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCTGGTGAGCTCAGATGACA |
| Suggested primer 2: | ACCAGCCACATAAGAAACCG |