Insertion junction: LMJ.RY0402.256129_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.256129
Chromosome:chromosome 4
Location:495066
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre04.g217930 (1 of 1) PF06911 - Senescence-associated protein (Senescence) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TAGTGTAGTGTGTTAGTGCCCCGTGCCTTG
Internal bar code:AGATCGTGTAATGGTTAAGTTC

Confirmation - LEAP-Seq

LEAP-Seq distance:251
LEAP-Seq percent confirming:99.3711
LEAP-Seq n confirming:158
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:8

Suggested primers for confirmation by PCR

Suggested primer 1:GTCGCGCGGTATCAGTTATT
Suggested primer 2:ACCCTCTACAACCCAGCCTT