| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.256153 |
| Chromosome: | chromosome 3 |
| Location: | 3958648 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g171700 | (1 of 1) 1.2.1.50 - Long-chain-fatty-acyl-CoA reductase / Acyl-CoA reductase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGAGACGGGAGGGGTGCAACCCGCTGCAGG |
| Internal bar code: | GATGGCCGCCGAAAAAGTACTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 305 |
| LEAP-Seq percent confirming: | 81.6667 |
| LEAP-Seq n confirming: | 245 |
| LEAP-Seq n nonconfirming: | 55 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AACTGCCCCAAACTATGCAC |
| Suggested primer 2: | ATTCCTTCCGTGTTGTTTGC |