| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.256175 |
| Chromosome: | chromosome 11 |
| Location: | 1185166 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g467690 | GST8B | (1 of 15) 2.5.1.18 - Glutathione transferase / S-(hydroxyalkyl)glutathione lyase; putative Glutathione S-transferase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGCCCACCACCCGGCCCCGCCGCCATCCT |
| Internal bar code: | ACTTCGGCGACGAACGTGGAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 364 |
| LEAP-Seq percent confirming: | 63.8889 |
| LEAP-Seq n confirming: | 46 |
| LEAP-Seq n nonconfirming: | 26 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATGAGGTCGCTAGCTCCAAA |
| Suggested primer 2: | GTAGCAACCTTCAAGGAGCG |