| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.256188 |
| Chromosome: | chromosome_2 |
| Location: | 4615993 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre02.g101050 | sense | intron |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | GTCCGCGGGGCAACCTCCCTGCCCCACACC |
| Internal bar code: | TGCCACACCTGCCGACCTCCAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 751 |
| LEAP-Seq percent confirming: | 87.1795 |
| LEAP-Seq n confirming: | 68 |
| LEAP-Seq n nonconfirming: | 10 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACAAGACCTTCACCGTCCAC |
| Suggested primer 2: | GCTCTTCTCCTCGCTCCTTT |