| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.256204 |
| Chromosome: | chromosome 1 |
| Location: | 5788875 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g041351 | (1 of 1) PTHR15967:SF0 - E2F-ASSOCIATED PHOSPHOPROTEIN | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTGTACCACTTCTACCACGTCTTCCCCAG |
| Internal bar code: | ATTAGCCGTCGGCGCCTCCCGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 498 |
| LEAP-Seq percent confirming: | 87.053 |
| LEAP-Seq n confirming: | 706 |
| LEAP-Seq n nonconfirming: | 105 |
| LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCACATCTCGGCTCACAGAC |
| Suggested primer 2: | TTTCTGACTCAGGCTTCGGT |