| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.256240 |
| Chromosome: | chromosome 6 |
| Location: | 1088687 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g256650 | (1 of 4) PTHR10887//PTHR10887:SF331 - DNA2/NAM7 HELICASE FAMILY // HELICASE MOV10L1-RELATED | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACCCGCCATCACCACCTCCGCCTGCTCCGC |
| Internal bar code: | GTCCTGTACGTCAGTACGCTTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 10 |
| LEAP-Seq percent confirming: | 83.871 |
| LEAP-Seq n confirming: | 26 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCATATACGGAAAGGCGTGT |
| Suggested primer 2: | TGCATAGACACCCTGAGCTG |