| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.256243 |
| Chromosome: | chromosome 16 |
| Location: | 2961423 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g664250 | OASTL2,ASL2 | O-acetylserine (Thiol)-lyase/cysteine synthase 2; (1 of 1) 4.2.1.22 - Cystathionine beta-synthase / Serine sulfhydrase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTGTAGCGTGGTGTAGCGCGCATGGCAGGT |
| Internal bar code: | AGCCGTTCGGAAGCCGTAGGGGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 483 |
| LEAP-Seq percent confirming: | 96.9945 |
| LEAP-Seq n confirming: | 355 |
| LEAP-Seq n nonconfirming: | 11 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AACAAGGTAGGTCTGGGGCT |
| Suggested primer 2: | ATGGAAGAAGGAGCGTGCTA |