| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.256247 |
| Chromosome: | chromosome 6 |
| Location: | 3002830 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g273650 | (1 of 3) 1.11.2.3 - Plant seed peroxygenase / Soybean peroxygenase | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCGTTGACACCGTATGGGTGCGACTTCTCC |
| Internal bar code: | CGTGTGTCATTATAACAATCAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 423 |
| LEAP-Seq percent confirming: | 99.6083 |
| LEAP-Seq n confirming: | 8391 |
| LEAP-Seq n nonconfirming: | 33 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGTCATTGTCTCTGCAGCAT |
| Suggested primer 2: | CAGGTCTCCAGTGCTCTTCC |