| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.256259 |
| Chromosome: | chromosome 7 |
| Location: | 4923569 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g346418 | (1 of 2) PTHR13271//PTHR13271:SF30 - UNCHARACTERIZED PUTATIVE METHYLTRANSFERASE // SUBFAMILY NOT NAMED | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCACCACCCGCAACCGCAACCCATACCGCC |
| Internal bar code: | TTTGGCCTCCTAGACCGCCCCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 387 |
| LEAP-Seq percent confirming: | 57.0836 |
| LEAP-Seq n confirming: | 963 |
| LEAP-Seq n nonconfirming: | 724 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGAAGGGGGTTTCTGTTGTA |
| Suggested primer 2: | ACAATCTCTTCATGCCTGGG |