| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.256262 |
| Chromosome: | chromosome 14 |
| Location: | 4042210 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g633626 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATCCCCTAACGGCTGGTTGGGCGTGCGACG |
| Internal bar code: | GATGACACGTTAGTGATTTCTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 671 |
| LEAP-Seq percent confirming: | 99.5775 |
| LEAP-Seq n confirming: | 9192 |
| LEAP-Seq n nonconfirming: | 39 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGTGGGAAATTAGCAAGCTC |
| Suggested primer 2: | TTGCTTCTGCACATCCAGTC |